Datasheet

FMR1 Antibody
CATALOG NUMBER: 7707

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:FMR1 antibody can be used for detection of FMR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 70 kDa

Observed: 74 kDa
SPECIFICITY:FMR1 antibody is human, mouse and rat reactive. Multiple isoforms of FMR1 are known to exist.
IMMUNOGEN:FMR1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FMR1.

The immunogen is located within the last 50 amino acids of FMR1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FMR1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FMR1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FMR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FMR1 Antibody: Fragile X mental retardation protein 1, FMRP
ACCESSION NO.:NP_002015
PROTEIN GI NO.:4503765
OFFICIAL SYMBOL:FMR1
GENE ID:2332

Background

BACKGROUND:Fragile X syndrome is a frequent form of inherited mental retardation caused by functional loss of the fragile X mental retardation protein, FMR1, also known as FMRP (1). FMR1 binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm (2). A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome (1). Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1) (3).
REFERENCES: 1) Jin P and Warren ST. Understanding the molecular basis of fragile X syndrome. Hum. Mol. Genet. 2000; 9:901-8.
2) Corbin F, Bouillon M, Fortin A, et al. The fragile X mental retardation protein is associated with poly(A)+ mRNA in actively translating polyribosomes. Hum. Mol. Genet. 1997; 1465-72.
3) Lu C, Lin L, Tan H, et al. Fragile X premutation RNA is sufficient to cause primary ovarian insufficiency in mice. Hum. Mol. Genet. 2012; 5039-47.

For Research Use Only