FMR1 Antibody Cat. No.: 7707

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: FMR1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FMR1.

The immunogen is located within the last 50 amino acids of FMR1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: FMR1 antibody can be used for detection of FMR1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: FMR1 antibody is human, mouse and rat reactive. Multiple isoforms of FMR1 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 70 kDa

Observed: 74 kDa

psi-iconProperties
PURIFICATION:FMR1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FMR1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FMR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:FMR1
ALTERNATE NAMES:FMR1 Antibody: Fragile X mental retardation protein 1, FMRP
ACCESSION NO.:NP_002015
PROTEIN GI NO.:4503765
GENE ID:2332
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Fragile X syndrome is a frequent form of inherited mental retardation caused by functional loss of the fragile X mental retardation protein, FMR1, also known as FMRP (1). FMR1 binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm (2). A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome (1). Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1) (3).
REFERENCES:1) Jin P and Warren ST. Understanding the molecular basis of fragile X syndrome. Hum. Mol. Genet. 2000; 9:901-8.
2) Corbin F, Bouillon M, Fortin A, et al. The fragile X mental retardation protein is associated with poly(A)+ mRNA in actively translating polyribosomes. Hum. Mol. Genet. 1997; 1465-72.
3) Lu C, Lin L, Tan H, et al. Fragile X premutation RNA is sufficient to cause primary ovarian insufficiency in mice. Hum. Mol. Genet. 2012; 5039-47.

ANTIBODIES FOR RESEARCH USE ONLY.

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