Datasheet

FLT3 Antibody
CATALOG NUMBER: 7027

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:FLT3 antibody can be used for detection of FLT3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1212 - 3T3 Cell Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide
PREDICTED MOLECULAR WEIGHT:109 kDa
SPECIFICITY:At least three isoforms of FLT3 are known to exist; this antibody will detect all isoforms.
IMMUNOGEN:FLT3 antibody was raised against an 18 amino acid synthetic peptide near the center of human FLT3.

The immunogen is located within amino acids 340 - 390 of FLT3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FLT3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FLT3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FLT3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FLT3 Antibody: FLK2, STK1, CD135, FLK-2, FLK2, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor
ACCESSION NO.:NP_004110
PROTEIN GI NO.:121114304
OFFICIAL SYMBOL:FLT3
GENE ID:2322

Background

BACKGROUND:FLT3 Antibody: FLT3 is a member of the class III receptor tyrosine kinase family and its internal tandem duplication (ITD) is preferentially seen in acute myeloid leukemia and myelodysplastic syndrome among various hematological malignancies. It is a membrane bound glycosylated protein that is expressed primarily in early hematopoietic progenitor cells. Upon binding its ligand FL, FLT3 activates multiple signaling pathways including the PI3K/Akt and Ras/MEK/MAPK pathways. In cells expressing FLT3-ITD, these pathways are constitutively on, resulting in abnormal cell growth and anti-apoptotic activity.
REFERENCES: 1) Rosnet O, Schiff C, Pebusque MJ, et al. Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells. Blood 1993; 82:1110-9
2) Yokoa S, Kiyoi H, Nakao M, et al. Internal tandem duplication of the FLT3 gene is preferentially seen in acute myeloid leukemia and myelodysplastic syndrome among various hematological malignancies. Leukemia 1997; 11:1605-9.
3) Takahashi S. Downstream molecular pathways of FLT3 in the pathogenesis of acute myeloid leukemia: biology and therapeutic implications. J. Hematol. and Oncol. 2011; 4:13.

For Research Use Only