Flotillin 1 Antibody Cat. No.: 45-051

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human
HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
IMMUNOGEN: The immunogen for this antibody is: C-SISQVNHKPLRTA
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in lysates of cell lines A431, Jurkat and K562 (calculated MW of 47.4kDa according to NP_005794.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_005794.1; NP_001305804.1).
POSITIVE CONTROL:1) H460 Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:FLOT1
ALTERNATE NAMES:FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae, DADB-118P11.2
ACCESSION NO.:NP_005794.1, NP_001305804.1
PROTEIN GI NO.:5031699
GENE ID:10211
psi-iconBackground and References
REFERENCES:1) Bickel PE, Scherer PE, Schnitzer JE, Oh P, Lisanti MP, Lodish HF. Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins. J Biol Chem. 1997 May 23;272(21):13793-802.

ANTIBODIES FOR RESEARCH USE ONLY.

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