FLIP Antibody Cat. No.: 2055

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.

The immunogen is located within amino acids 180 - 230 of FLIP.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: FLIP can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kDa and 25 kDa bands can be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: Antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT: 35 kDa and 25 kDa

psi-iconProperties
PURIFICATION:FLIP Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/ml
STORAGE CONDITIONS:FLIP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CFLAR
ALTERNATE NAMES:FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Casper, FLAME1, c-FLIP, FLAME-1, I-FLICE, c-FLIPL, c-FLIPR, c-FLIPS, CASP8AP1, CASH, CASP8 and FADD-like apoptosis regulator, Caspase homolog
ACCESSION NO.:AAC51622
PROTEIN GI NO.:2253679
GENE ID:8837
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:FLIP Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively. The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and usurpin. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively.
REFERENCES:1) Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521
2) Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195
3) Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763
4) Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257

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