FLI1 Antibody Cat. No.: 7449

Availability: In stock

$0.00

 Price Options

* Required Fields

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
IMMUNOGEN: FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1.

The immunogen is located within the last 50 amino acids of FLI1 .
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: FLI1 antibody can be used for detection of FLI1 by Western blot at 0.5 - 1 μg/ml.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: FLI1 antibody is human, mouse and rat reactive. At least four isoforms of FLI1 are known to exist; this antibody will detect all four.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 50 kDa

Observed: 52kDa

psi-iconProperties
PURIFICATION:FLI1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FLI1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FLI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:FLI1
ALTERNATE NAMES:FLI1 Antibody: EWSR2, SIC-1, BDPLT21
ACCESSION NO.:NP_002008
PROTEIN GI NO.:7110593
GENE ID:2313
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The Friend leukemia virus integration 1 (FLI1) protein is a transcription factor containing an ETS DNA-binding domain and is highly expressed in hematopoietic cell lineages and vascular endothelial cells (1). FLI1 plays important roles in megakaryocytic differentiation (2) and vascular homeostasis (3). The FLI1 gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. (4). Abnormal expression of FLI1 can be used as an adverse prognostic indicator for acute myeloid leukemia (5).
REFERENCES:1) Hollenhorst PC, Jones DA, and Graves BJ. Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors. Nuc. Acids Res. 2004; 32:5693-702.
2) Spyropoulos DD, Pharr PN, Lavenburg KR, et al. Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. Mol. Cell Biol. 2000; 20:5643-52.
3) Asano Y, Stawski L, Hant F, et al. Endothelial Fli1 deficiency impairs vascular homeostasis: a role in scleroderma vasculopathy. Am. J. Pathol. 2010; 176:1983-98.
4) May WA, Gishizky ML, Lessnick SL, et al. Ewing sarcoma 11;22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation. Proc. Natl. Acad. Sci. USA 1993; 90:5752-6. 5. Kornblau SM, Qiu YH, Zhang N, et al. Abnormal expression of FLI1 protein is an adverse prognostic factor in acute myeloid leukemia. Blood 2011; 118:5604-12.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.