| SPECIES: | Human, Mouse |
| SOURCE SPECIES: | Salmonella typhimurium strain 14028 |
| TESTED APPLICATIONS: | |
| APPLICATIONS: | This recombinant proteins is for research use only. |
| BIOLOGICAL ACTIVITY: | Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1ug /mouse. |
Specifications| PURITY: | >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| PHYSICAL STATE: | Lyophilized |
| BUFFER: | Contains PBS. Reconstitute with 100 μl sterile water. |
| CONCENTRATION: | 0.1mg/ml after reconstitution. |
| STORAGE CONDITIONS: | Stable for at least 6 months after receipt when stored at -20˚C. |
| ALTERNATE NAMES: | FliC |
| ACCESSION NO.: | P06179 |
| PROTEIN GI NO.: | 20141325 |
| OFFICIAL SYMBOL: | fliC |
| GENE ID: | 1253480 |
| BACKGROUND: | Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4). |
FOR RESEARCH USE ONLY.
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