FHIT Antibody Cat. No.: 7455

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (88%), Rat: (72%)
IMMUNOGEN: FHIT antibody was raised against an 18 amino acid peptide near the carboxy terminus of human FHIT.

The immunogen is located within the last 50 amino acids of FHIT .
TESTED APPLICATIONS: ELISA, IF, WB
APPLICATIONS: FHIT antibody can be used for detection of FHIT by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: FHIT antibody is human and mouse reactive.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 16 kDa

Observed: 15kDa

psi-iconProperties
PURIFICATION:FHIT Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FHIT Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FHIT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:FHIT
ALTERNATE NAMES:FHIT Antibody : FRA3B, AP3AaseAP3A hydrolase, AP3Aase
ACCESSION NO.:NP_002003
PROTEIN GI NO.:4503719
GENE ID:2272
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:FHIT is member of the histidine triad gene family and is a diadenosine involved in purine metabolism (1). FHIT is also thought to be a tumor suppressor gene and is involved in multiple apoptotic pathways (1,2). The FHIT gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene (3). Aberrant transcripts from this gene have been found in multiple carcinomas (4).
REFERENCES:1) Barnes LD, Garrison PN, Siprashvili Z, et al. Fhit, a putative tumor suppressor in humans, is a dinucleotide 5',5'''-P1,P3-triphosphate hydrolase. Biochemistry 1996; 35:11529-35.
2) Wali A. FHIT: doubts are clear now. ScientificWorldJournal 2010; 10:1142-51.
3) Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell 1996; 84:587-97.
4) Drusco A, Pekarsky Y, Costinean S, et al. Common fragile site tumor suppressor genes and corresponding mouse models of cancer. J. Biomed. Biotechnol.2011; Epub 2010 Dec 29.

ANTIBODIES FOR RESEARCH USE ONLY.

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