Datasheet

FHIT Antibody
CATALOG NUMBER: 7455

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (88%), Rat: (72%)
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:FHIT antibody can be used for detection of FHIT by Western blot at 1 - 2 μg/ml.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 16 kDa

Observed: 15kDa
SPECIFICITY:FHIT antibody is human and mouse reactive.
IMMUNOGEN:FHIT antibody was raised against an 18 amino acid peptide near the carboxy terminus of human FHIT.

The immunogen is located within the last 50 amino acids of FHIT .
HOST SPECIES:Rabbit

Properties

PURIFICATION:FHIT Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FHIT Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FHIT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FHIT Antibody : FRA3B, AP3AaseAP3A hydrolase, AP3Aase
ACCESSION NO.:NP_002003
PROTEIN GI NO.:4503719
OFFICIAL SYMBOL:FHIT
GENE ID:2272

Background

BACKGROUND:FHIT is member of the histidine triad gene family and is a diadenosine involved in purine metabolism (1). FHIT is also thought to be a tumor suppressor gene and is involved in multiple apoptotic pathways (1,2). The FHIT gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene (3). Aberrant transcripts from this gene have been found in multiple carcinomas (4).
REFERENCES: 1) Barnes LD, Garrison PN, Siprashvili Z, et al. Fhit, a putative tumor suppressor in humans, is a dinucleotide 5',5'''-P1,P3-triphosphate hydrolase. Biochemistry 1996; 35:11529-35.
2) Wali A. FHIT: doubts are clear now. ScientificWorldJournal 2010; 10:1142-51.
3) Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell 1996; 84:587-97.
4) Drusco A, Pekarsky Y, Costinean S, et al. Common fragile site tumor suppressor genes and corresponding mouse models of cancer. J. Biomed. Biotechnol.2011; Epub 2010 Dec 29.

For Research Use Only