Datasheet

FGFR3 Antibody
CATALOG NUMBER: 7425

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (95%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:FGF3 antibody can be used for detection of FGFR3 by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 89 kDa

Observed: 9+2, 98 kDa
SPECIFICITY:FGFR3 antibody is human, mouse and rat reactive. At least three isoforms of FGFR3 are known to exist; this antibody will detect all three.
IMMUNOGEN:FGFR3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FGFR3.

The immunogen is located within amino acids 630 - 680 of FGFR3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FGFR3 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FGFR3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FGFR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FGFR3 Antibody: ACH, CEK2, JTK4, CD333, HSFGFR3EX, Fibroblast growth factor receptor 3, FGFR-3
ACCESSION NO.:NP_001156685
PROTEIN GI NO.:254028242
OFFICIAL SYMBOL:FGFR3
GENE ID:2261

Background

BACKGROUND:FGFR3 Antibody: FGFR3 is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis (1). It is required for normal skeleton development and promotes apoptosis in chondrocytes and cancer cell proliferation. FGFR3 protein contains three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain (1,2). It is alternatively spliced to produce three isoforms that are expressed in brain, kidney and testis. Defects in FGFR3 are associated with several diseases, including achondroplasia and hypochondroplasia (3,4). Mutations in FGFR3 are also a cause of some bladder and cervical cancers (5).
REFERENCES: 1) Keegan K, Johnson DE, and Williams LT. Isolation of an additional member of the fibroblast growth factor receptor family, FGFR-3. Proc. Natl. Acad. Sci. USA 1991; 88:1095-9.
2) Turner N and Grose R. Fibroblast growth factor signalling: from development to cancer. Nat. Rev. Cancer 2010; 10:116-29.
3) Superti-Furga A, Eich G, Bucher HU, et al. A glycine 375-to-cysteine substitution in the transmembrane domain of the fibroblast growth factor receptor-3 in a newborn with achondroplasia. Eur. J. Pediatr. 1995; 154:215-9.
4) Bellus GA, McIntosh I, Smith EA, et al. A recurrent mutation in the tyrosine kinase domain of fibroblast growth factor receptor 3 causes hypochondroplasia. Nat. Genet. 1995; 10:357-9.

For Research Use Only