Datasheet

FEZ2 Antibody
CATALOG NUMBER: 4403

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:FEZ2 antibody can be used for the detection of FEZ2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
IMMUNOGEN:FEZ2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FEZ2.

The immunogen is located within the last 50 amino acids of FEZ2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FEZ2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FEZ2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FEZ2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FEZ2 Antibody: HUM3CL, Fasciculation and elongation protein zeta-2, Zygin II
ACCESSION NO.:NP_001036013
PROTEIN GI NO.:110349756
OFFICIAL SYMBOL:FEZ2
GENE ID:9637

Background

BACKGROUND:FEZ2 Antibody: Fasciculation and elongation protein zeta-2 (FEZ2) is a homolog to the mammalian FEZ1, itself an ortholog of the C. elegans UNC-76. In contrast to FEZ1, FEZ2 mRNA is widely expressed in mouse tissues. FEZ2 interacts with protein kinase C (PKC)-zeta substrate and induces neurite extension of PC12 cells when co-expressed with a constitutively active form of PKC-zeta, suggesting FEZ2 may play an important role in the morphological changes of various cells by associating with PKC-zeta in a tissue non-specific manner. FEZ2 can interact with FEZ1 through its c-terminal regions and especially its coiled-coil region. At least two isoforms of FEZ2 are known to exist.
REFERENCES: 1) Fujita T, Ikuta J, Okajima T, et al. Identification of a tissue-non-specific homologue of axonal fasciculation and elongation protein zeta-1. Biochem. Biophys. Res. Commun.2004; 313:738-44.
2) Kuroda S, Nakagawa N, Tokunaga C, et al. Mammalian homologue of the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth is a protein kinase C z-interacting protein. J. Cell Biol.1999; 144:403-11.
3) Assmann EM, Alborghetti MR, Camargo MER, et al. FEZ1 dimerization and interaction with transcription regulatory proteins involves its coiled-coil region. J. Biol. Chem.2006; 281:9869-81.

For Research Use Only