FEZ1 Antibody Cat. No.: 4265

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: FEZ1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FEZ1.

The immunogen is located within amino acids 270 - 320 of FEZ1.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: FEZ1 antibody can be used for the detection of FEZ1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, FEZ1 will often run at higher molecular weight in SDS-PAGE.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 42 kDa

Observed: 46 kDa

psi-iconProperties
PURIFICATION:FEZ1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FEZ1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FEZ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:FEZ1
ALTERNATE NAMES:FEZ1 Antibody: Fasciculation and elongation protein zeta-1, Zygin I
ACCESSION NO.:Q99689
PROTEIN GI NO.:13431526
GENE ID:9638
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:FEZ1 Antibody: Similar to its C. elegans homolog UNC-76, mammalian FEZ1, an abundant membrane protein and protein kinase C (PKC)-Z substrate, is involved in axonal guidance. Following phosphorylation by PKC-Z, FEZ1 translocates from the plasma membrane to the cytoplasm. FEZ1 is then able to interact with a number of proteins involved in axonal outgrowth and cellular transport such as DISC1, a candidate gene for schizophrenia, as well as kinesin-1, a microtubule-based motor protein. FEZ1 also interacts with proteins involved in transcriptional regulation and chromatin remodeling, suggesting that FEZ1 may have nuclear regulatory functions in addition to those for neuronal development. At least two distinct isoforms of FEZ1 are known to exist.
REFERENCES:1) Kuroda S, Nakagawa N, Tokunaga C, et al. Mammalian homologue of the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth is a protein kinase C z-interacting protein. J. Cell Biol.1999; 144:403-11.
2) Bloom L and Horvitz HR. The Caenorhabditis elegans gene unc-76 and its human homologs define a new gene family involved in axonal outgrowth and fasciculation. Proc. Natl. Acad. Sci. USA1997; 88:9578-82.
3) Miyohi K, Honda A, Baba K, et al. Disrupted-in-schizophrenia 1, a candidate gene for schizophrenia, participates in neurite outgrowth. Mol. Psychiatry2003; 8:685-94.
4) Blasius TL, Cai D, Jih GT, et al. Two binding partners cooperate to activate the molecular motor kinesin-1. J. Cell Biol.2007; 176:11-7.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.