Datasheet

FEZ1 Antibody
CATALOG NUMBER: 4265

Availability: In stock

$0.00
Product Price Options
$0.00
Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:FEZ1 antibody can be used for the detection of FEZ1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, FEZ1 will often run at higher molecular weight in SDS-PAGE.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1207 - Raji Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 42 kDa

Observed: 46 kDa
IMMUNOGEN:FEZ1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FEZ1.

The immunogen is located within amino acids 270 - 320 of FEZ1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FEZ1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FEZ1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FEZ1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FEZ1 Antibody: Fasciculation and elongation protein zeta-1, Zygin I
ACCESSION NO.:Q99689
PROTEIN GI NO.:13431526
OFFICIAL SYMBOL:FEZ1
GENE ID:9638

Background

BACKGROUND:FEZ1 Antibody: Similar to its C. elegans homolog UNC-76, mammalian FEZ1, an abundant membrane protein and protein kinase C (PKC)-Z substrate, is involved in axonal guidance. Following phosphorylation by PKC-Z, FEZ1 translocates from the plasma membrane to the cytoplasm. FEZ1 is then able to interact with a number of proteins involved in axonal outgrowth and cellular transport such as DISC1, a candidate gene for schizophrenia, as well as kinesin-1, a microtubule-based motor protein. FEZ1 also interacts with proteins involved in transcriptional regulation and chromatin remodeling, suggesting that FEZ1 may have nuclear regulatory functions in addition to those for neuronal development. At least two distinct isoforms of FEZ1 are known to exist.
REFERENCES: 1) Kuroda S, Nakagawa N, Tokunaga C, et al. Mammalian homologue of the Caenorhabditis elegans UNC-76 protein involved in axonal outgrowth is a protein kinase C z-interacting protein. J. Cell Biol.1999; 144:403-11.
2) Bloom L and Horvitz HR. The Caenorhabditis elegans gene unc-76 and its human homologs define a new gene family involved in axonal outgrowth and fasciculation. Proc. Natl. Acad. Sci. USA1997; 88:9578-82.
3) Miyohi K, Honda A, Baba K, et al. Disrupted-in-schizophrenia 1, a candidate gene for schizophrenia, participates in neurite outgrowth. Mol. Psychiatry2003; 8:685-94.
4) Blasius TL, Cai D, Jih GT, et al. Two binding partners cooperate to activate the molecular motor kinesin-1. J. Cell Biol.2007; 176:11-7.

For Research Use Only