Datasheet

FCHO2 Antibody
CATALOG NUMBER: 6871

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:IF
APPLICATIONS:FCHO2 antibody can be used for detection of FCHO2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1461 - Rat Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
SPECIFICITY:FCHO2 antibody is predicted to not cross-react with other FCHO protein family members. At least the isoforms of FCHO2 are known to exist; this antibody will detect only the two longest isoforms.
IMMUNOGEN:FCHO2 antibody was raised against a 15 amino acid synthetic peptide near the center of human FCHO2.

The immunogen is located within amino acids 340 - 390 of FCHO2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FCHO2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FCHO2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FCHO2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FCHO2 Antibody: FCH domain only protein 2
ACCESSION NO.:NP_620137
PROTEIN GI NO.:226371723
OFFICIAL SYMBOL:FCHO2
GENE ID:115548

Background

BACKGROUND:FCHO2 Antibody: FCHO2 (FCH domain only 2) is a ubiquitously expressed member of the FCFBS superfamily characterized by FES-CIP4 homology (FCH) domain, an FBH domain, and an SH3 domain. Both FCHO2 and the related protein FCHO1 are mediators of clathrin-mediated endocytosis, acting to sculpt the initial bud site and recruit the clathrin machinery for clathrin-coated vesicle formation. FCHO2 binds to Eps15, an important adaptor protein in clathrin-mediated endocytosis.
REFERENCES: 1) Katoh M and Katoh M. Identification and characterization of human FCHO2 and mouse fcho2 genes in silico. Int. J. Mol. Med. 2004; 14:327-31
2) Henne WM, Boucrot E, Meinecke M, et al. FCHo proteins are nucleators of clathrin-mediated endocytosis. Science 2010; 328:1281-4.
3) Uezu A, Umeda K, Tsujita K, et al. Characterization of the EFC/F-BAR domain protein, FCHO2. Genes Cells 2011; 16:868-78.

For Research Use Only