Specifications
| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: |
Human, Mouse, Rat |
| IMMUNOGEN: |
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXO28. |
| TESTED APPLICATIONS: |
ELISA, WB |
| APPLICATIONS: |
FBXO28 antibody can be used for detection of FBXO28 by ELISA at 1:312500. FBXO28 antibody can be used for detection of FBXO28 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| POSITIVE CONTROL: | 1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate |
| PREDICTED MOLECULAR WEIGHT: |
41 kDa |
Properties
| PURIFICATION: | Antibody is purified by peptide affinity chromatography method. |
| CLONALITY: | Polyclonal |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| CONCENTRATION: | batch dependent |
| STORAGE CONDITIONS: | For short periods of storage (days) store at 4˚C. For longer periods of storage, store FBXO28 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
Additional Info
| OFFICIAL SYMBOL: | FBXO28 |
| ALTERNATE NAMES: | FBXO28, FLJ10766, Fbx28, KIAA0483, CENP-30 |
| ACCESSION NO.: | NP_055991 |
| PROTEIN GI NO.: | 7662158 |
| GENE ID: | 23219 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
Background and References
| BACKGROUND: | Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains.Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]). |
| REFERENCES: | 1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
ANTIBODIES FOR RESEARCH USE ONLY.
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