Fas Ligand Antibody (biotin) Cat. No.: XP-5130Bt

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human
IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL/Apo1 (human FasL/Apo1).
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ELISA:

Sandwich: To detect hFasL/Apo1L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FasL/Apo1L (XP-5130) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFasL/Apo1L.

Western Blot:

To detect hFasL/Apo1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL/Apo1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

psi-iconProperties
PURIFICATION:Anti-hFasL/Apo1 specific antibody was purified by affinity chromatography and then biotinylated.
CLONALITY:Polyclonal
CONJUGATE:Biotin
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:Fas Ligand antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:FASLG
ALTERNATE NAMES:APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, APT1LG1, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL
ACCESSION NO.:P48023
PROTEIN GI NO.:1345957
GENE ID:356
USER NOTE:Centrifuge vial prior to opening.
psi-iconBackground and References
BACKGROUND:Fas Ligand is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.

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