FAM59A Antibody Cat. No.: 5479

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: FAM59A antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAM59A.

The immunogen is located within amino acids 630 - 680 of FAM59A.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: FAM59A antibody can be used for detection of FAM59A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide

psi-iconProperties
PURIFICATION:FAM59A Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:FAM59A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FAM59A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:FAM59A
ALTERNATE NAMES:FAM59A Antibody: Gm944, FAM59A, C18orf11, GRB2-associated and regulator of MAPK protein, GRB2-associated and regulator of MAPK1
ACCESSION NO.:Q9H706
PROTEIN GI NO.:125991851
GENE ID:64762
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:FAM59A Antibody: FAM59A, also known as GAREM, was initially identified through a mass spectroscoptic method that measures different phosphotyrosine states of proteins in response to different cell stimuli. FAM59A is a downstream molecule in the EGF signaling pathway that is tyrosine-phosphorylated, and this phosphorylation is needed for the binding of Grb2, an adaptor protein crucial to the transduction of growth signals from the plasma membrane to the nucleus. ERK activation in response to EGF stimulation is regulated by FAM59A in COS-7 and HeLa cells, and the overexpression of FAM59A stimulated cell proliferation and colony formation in soft agar, suggesting that FAM59A might be a critical protein with roles in ligand-mediated signaling pathway of the EGF receptor and the tumorigenesis of cells.
REFERENCES:1) Blagoev B, Ong SE, Kratchmarova I, et al. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat. Biotechnol.2004; 22:1139-45.
2) Tashiro K, Tsunematsu T, Okubo H, et al. GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through activation of extracellular signal-regulated kinase signaling. J. Biol. Chem.2009; 284:20206-14.

ANTIBODIES FOR RESEARCH USE ONLY.

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