Specifications
Properties
Additional Info
Background
Specifications
| SPECIES REACTIVITY: | Human, Mouse, Rat |
|---|---|
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | FAM120A antibody can be used for detection of FAM120A by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| POSITIVE CONTROL: | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
| SPECIFICITY: | This antibody will not cross-reacti with FAM120B / PGCC1. |
| IMMUNOGEN: | FAM120A antibody was raised against an 18 amino acid synthetic peptide from near the center of human FAM120A. The immunogen is located within amino acids 610 - 660 of FAM120A. |
| HOST SPECIES: | Rabbit |
Properties
| PURIFICATION: | FAM120A Antibody is affinity chromatography purified via peptide column. |
|---|---|
| PHYSICAL STATE: | Liquid |
| BUFFER: | FAM120A Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | FAM120A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
Additional Info
| ALTERNATE NAMES: | FAM120A Antibody: OSSA, C9orf10, DNAPTP1, DNAPTP5, KIAA0183, OSSA, Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated Src activator |
|---|---|
| ACCESSION NO.: | NP_055427 |
| PROTEIN GI NO.: | 39652628 |
| OFFICIAL SYMBOL: | FAM120A |
| GENE ID: | 23196 |
Background
| BACKGROUND: | FAM120A Antibody: FAM120A (C9orf10) is a member of the constitutive coactivator of PPAR gamma family and the gene was mapped to chromosome 9q22.31. FAM120A was recently detected within the Pur-alpha-containing mRNA-protein complex in the brain. As a novel RNA-binding protein, FAM120A is a critical component of the oxidative stress-induced survival signaling. It may participate in mRNA transport in the cytoplasm. FAM120A activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. FAM120A protects cells from apoptosis through activation of SRKs in response to oxidative stress. Blocking of the survival signaling mediated by FAM120A, which sensitizes the cancer cells to stress-induced apoptosis, may be a novel therapeutic approach for gastric scirrhous carcinoma cells. |
|---|---|
| REFERENCES: | 1) Holden S and Raymond FL. The human gene CXorf17 encodes a member of a novel family of putative transmembrane proteins: cDNA cloning and characterization of CXorf17 and its mouse ortholog orf34. Gene2003; 318:149-61. | 2) Kobayashi Y, Suzuki K, Kobayashi H, et al. C9orf10 protein, a novel protein component of Puralpha-containing mRNA-protein particles (Puralpha-mRNPs): characterization of developmental and regional expressions in the mouse brain. J. Histochem. Cytochem.2008; 56:723-31. | 3) Tanaka M, Sasaki K, Kamata R, et al. A novel RNA-binding protein, Ossa/C9orf10, regulates activity of Src kinases to protect cells from oxidative stress-induced apoptosis. Mol. Cell. Biol.2009; 29:402-13. |
For Research Use Only
