Datasheet

FAM120A Antibody
CATALOG NUMBER: 5307

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:FAM120A antibody can be used for detection of FAM120A by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
SPECIFICITY:This antibody will not cross-reacti with FAM120B / PGCC1.
IMMUNOGEN:FAM120A antibody was raised against an 18 amino acid synthetic peptide from near the center of human FAM120A.

The immunogen is located within amino acids 610 - 660 of FAM120A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FAM120A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FAM120A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:FAM120A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FAM120A Antibody: OSSA, C9orf10, DNAPTP1, DNAPTP5, KIAA0183, OSSA, Constitutive coactivator of PPAR-gamma-like protein 1, Oxidative stress-associated Src activator
ACCESSION NO.:NP_055427
PROTEIN GI NO.:39652628
OFFICIAL SYMBOL:FAM120A
GENE ID:23196

Background

BACKGROUND:FAM120A Antibody: FAM120A (C9orf10) is a member of the constitutive coactivator of PPAR gamma family and the gene was mapped to chromosome 9q22.31. FAM120A was recently detected within the Pur-alpha-containing mRNA-protein complex in the brain. As a novel RNA-binding protein, FAM120A is a critical component of the oxidative stress-induced survival signaling. It may participate in mRNA transport in the cytoplasm. FAM120A activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. FAM120A protects cells from apoptosis through activation of SRKs in response to oxidative stress. Blocking of the survival signaling mediated by FAM120A, which sensitizes the cancer cells to stress-induced apoptosis, may be a novel therapeutic approach for gastric scirrhous carcinoma cells.
REFERENCES: 1) Holden S and Raymond FL. The human gene CXorf17 encodes a member of a novel family of putative transmembrane proteins: cDNA cloning and characterization of CXorf17 and its mouse ortholog orf34. Gene2003; 318:149-61.
2) Kobayashi Y, Suzuki K, Kobayashi H, et al. C9orf10 protein, a novel protein component of Puralpha-containing mRNA-protein particles (Puralpha-mRNPs): characterization of developmental and regional expressions in the mouse brain. J. Histochem. Cytochem.2008; 56:723-31.
3) Tanaka M, Sasaki K, Kamata R, et al. A novel RNA-binding protein, Ossa/C9orf10, regulates activity of Src kinases to protect cells from oxidative stress-induced apoptosis. Mol. Cell. Biol.2009; 29:402-13.

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