Datasheet

FAIM2 Antibody
CATALOG NUMBER: 2285

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (94%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:FAIM2 antibody can be used for detection of FAIM2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 1402 - Mouse Lung Tissue Lysate
IMMUNOGEN:FAIM2 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human FAIM2.

The immunogen is located within the first 50 amino acids of FAIM2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:FAIM2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:FAIM2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:FAIM2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:FAIM2 Antibody: LFG, LFG2, NGP35, NMP35, TMBIM2, KIAA0950, LFG, Protein lifeguard 2, Fas apoptotic inhibitory molecule 2
ACCESSION NO.:AAF06327
PROTEIN GI NO.:6273281
OFFICIAL SYMBOL:FAIM2
GENE ID:23017

Background

BACKGROUND:FAIM2 Antibody: Programmed cell death regulates a number of biological processes such as normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. FAIM2 is a recently identified protein that can inhibit the apoptotic signal transduced by the Fas receptor but not from the related tumor necrosis factor-alpha death signal. In this respect, FAIM2 is functionally similar to the anti-apoptotic proteins FAIM, FLIP and Bcl-xL. FAIM2, a seven membrane spanning protein, can bind the Fas receptor but does not regulate Fas expression or inhibit binding of FADD to Fas. FAIM2 is widely distributed, but highly expressed in the hippocampus and other neural tissues. FAIM2 was also identified as the neural membrane protein 35 (NMP35) and its expression is known to be regulated by the Phosphatidylinositol 3-kinase-Akt/PKB pathway.
REFERENCES: 1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2) Somia NV, Schmitt MJ, Vetter DE, et al. FAIM2: an anti-apoptotic gene that provides protection from Fas-mediated cell death. Proc. Natl. Acad. Sci. USA 1999; 96:12667-72.
3) Schneider TJ, Fischer GM, Donohoe TJ, et al. A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes. J. Exp. Med. 1999; 189:949-55.
4) Schweitzer B, Taylor V, Welcher AA, et al. Neural membrane protein 35 (NMP35): a novel member of a gene family which is highly expressed in the adult nervous system. Mol. Cell. Neurosci. 1998; 11:260-73.

For Research Use Only