Datasheet

F4/80 Antigen Antibody [BM8.1] (PerCP-Cy5.5)
CATALOG NUMBER: 76-062

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Mouse
TESTED APPLICATIONS:Flow
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
SPECIFICITY:The BM8.1 monoclonal antibody specifically binds to the Mouse125 kDa F4/80 antigen, expressed by most mature macrophages.
HOST SPECIES:Rat

Properties

PURIFICATION:The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
PHYSICAL STATE:liquid
BUFFER:Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:The product should be stored undiluted at 4˚C and should be protected from prolonged exposure to light. Do not freeze.
CLONALITY:Monoclonal
ISOTYPE:Rat IgG2a, kappa
CONJUGATE:PerCP-Cy5.5

Additional Info

ALTERNATE NAMES:Ly71, F4/80, Gpf480, TM7LN3, DD7A5-7, EGF-TM7, Emr1
OFFICIAL SYMBOL:Emr1
GENE ID:13733

Background

BACKGROUND:The BM8.1 monoclonal antibody specifically binds to the Mouse125 kDa F4/80 antigen, expressed by most mature macrophages. F4/80 is a transmembrane protein used as a marker of macrophages, although it is also expressed on Kupffer and Langerhans cells. The expression of F4/80 antigen is upregulated on bone marrow cells stimulated in vitro with the macrophage colony stimulating factor. The F4/80 antigen is a requirement for the induction of CD8 T cells-mediated peripheral tolerance.
REFERENCES: 1) Zwadlo, G., Brcker, E. B., Von Bassewitz, D. B., Feige, U., Sorg, C. (1985). A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes.The Journal of Immunology,134(3), 1487-1492.
2) Leenen, P. J., de Bruijn, M. F., Voerman, J. S., Campbell, P. A., van Ewijk, W. (1994). Markers of mouse macrophage development detected by monoclonal antibodies.Journal of immunological methods,174(1), 5-19.
3) Schaller, E., Macfarlane, A. J., Rupec, R. A., Gordon, S., McKnight, A. J., Pfeffer, K. (2002). Inactivation of the F4/80 glycoprotein in the mouse germ line.Molecular and cellular biology,22(22), 8035-8043.

For Research Use Only