Datasheet

EZH2 Antibody
CATALOG NUMBER: 6263

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:EZH2 antibody can be used for detection of EZH2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
SPECIFICITY:Multiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1.
IMMUNOGEN:EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2.

The immunogen is located within amino acids 190 - 240 of EZH2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:EZH2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:EZH2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EZH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:EZH2 Antibody: WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A, Histone-lysine N-methyltransferase EZH2
ACCESSION NO.:AAH10858
PROTEIN GI NO.:21361095
OFFICIAL SYMBOL:EZH2
GENE ID:2146

Background

BACKGROUND:EZH2 Antibody: EZH2 was initially identified as a homolog of the drosophila Enhancer of Zeste through exon trap screening of chromosome 21. Both EZH2 and the related protein EZH1 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair. Overexpression of EZH2 has been reported as a marker for advanced and metastatic cancers.
REFERENCES: 1) Chen H, Rossier C, and Antonarakis SE. Cloning of a human homolog of the Drosophila enhancer of zeste gene (EZH2) that maps to chromosome 21q22.2. Genomics 1996; 38:30-7
2) Margueron R, Li G, Sarma K, et al. Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol. Cell 2008; 32:503-18.
3) Shen X, Liu Y, Hsu YJ, et al. EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol. Cell 2008; 32:491-502.
4) Ezhkova E, Lien WH, Stokes N, et al. EZH1 and EZH2 cogovern histone H3K27 trimethylation and are essential for hair follicle homeostasis and wound repair. Genes Dev. 2011; 25:485-98.

For Research Use Only