Datasheet

ETV4 Antibody
CATALOG NUMBER: 8535

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Zebrafish: (72%)
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:ETV4 antibody can be used for Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 1202 - A431 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 53 kDa

Observed: 53 kDa
SPECIFICITY:At least three isoforms of ETV4 are known to exist. This antibody will only detect the two longest isoforms.
IMMUNOGEN:ETV4 antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human ETV4.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ETV4 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ETV4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ETV4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ETV4 Antibody: ETS variant 4, E1AF, PEA3, E1A-F, PEAS3
ACCESSION NO.:NP_001977
PROTEIN GI NO.:24307883
OFFICIAL SYMBOL:ETV4
GENE ID:2118

Background

BACKGROUND:ETV4 Antibody: ETV4, along with the related proteins ETV1 and ETV5, form the PEA3 subfamily of the E26 transformation-specific (ETS) transcription factor family (1). ETV4 is thought to play roles in embryonic kidney development and fertility (2,3), and is also thought to be an oncoprotein in multiple cancers (4).
REFERENCES: 1) Xin JH, Cowie A, LAchance P, et al. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells. Genes Dev. 1992; 6:481-96.
2) Lu BC, Cebrian C, Chi X, et al. Etv4 and Etv5 are required downstream of GDNF and Ret for kidney branching morphogenesis. Nat. Genet. 2009; 41:1295-302.
3) Laing MA, Coonrod S, Hinton BT, et al. Male sexual dysfunction in mice bearing targeted mutant alleles of the PEA3 ets gene. Mol. Cell. Biol. 2000; 20:9337-45.
4) Oh S, Shin S, and Janknecht R. ETV1, 4 and 5: An oncogenic subfamily of ETS transcription factors. Biochim. Biophys. Acta 2012: 1826:1-12.

For Research Use Only