ETV4 Antibody Cat. No.: 8535

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Zebrafish: (72%)
IMMUNOGEN: ETV4 antibody was raised against a peptide corresponding to 19 amino acids near the amino terminus of human ETV4.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ETV4 antibody can be used for Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: At least three isoforms of ETV4 are known to exist. This antibody will only detect the two longest isoforms.
POSITIVE CONTROL:1) Cat. No. 1303 - Human Brain Tissue Lysate
 2) Cat. No. 1202 - A431 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 53 kDa

Observed: 53 kDa

psi-iconProperties
PURIFICATION:ETV4 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ETV4 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ETV4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ETV4
ALTERNATE NAMES:ETV4 Antibody: ETS variant 4, E1AF, PEA3, E1A-F, PEAS3
ACCESSION NO.:NP_001977
PROTEIN GI NO.:24307883
GENE ID:2118
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ETV4 Antibody: ETV4, along with the related proteins ETV1 and ETV5, form the PEA3 subfamily of the E26 transformation-specific (ETS) transcription factor family (1). ETV4 is thought to play roles in embryonic kidney development and fertility (2,3), and is also thought to be an oncoprotein in multiple cancers (4).
REFERENCES:1) Xin JH, Cowie A, LAchance P, et al. Molecular cloning and characterization of PEA3, a new member of the Ets oncogene family that is differentially expressed in mouse embryonic cells. Genes Dev. 1992; 6:481-96.
2) Lu BC, Cebrian C, Chi X, et al. Etv4 and Etv5 are required downstream of GDNF and Ret for kidney branching morphogenesis. Nat. Genet. 2009; 41:1295-302.
3) Laing MA, Coonrod S, Hinton BT, et al. Male sexual dysfunction in mice bearing targeted mutant alleles of the PEA3 ets gene. Mol. Cell. Biol. 2000; 20:9337-45.
4) Oh S, Shin S, and Janknecht R. ETV1, 4 and 5: An oncogenic subfamily of ETS transcription factors. Biochim. Biophys. Acta 2012: 1826:1-12.

ANTIBODIES FOR RESEARCH USE ONLY.

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