Datasheet

ESX1 Antibody
CATALOG NUMBER: 5597

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Rat: (71%), Mouse: (71%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ESX1 antibody can be used for detection of ESX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1313 - Human Testis Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
IMMUNOGEN:ESX1 antibody was raised against a 19 amino acid synthetic peptide near the center of human ESX1.

The immunogen is located within amino acids 140 - 190 of ESX1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ESX1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ESX1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ESX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ESX1 Antibody: ESX1L, ESXR1, ESX1L, ESX1R, Homeobox protein ESX1, Extraembryonic, spermatogenesis, homeobox 1
ACCESSION NO.:Q8N693
PROTEIN GI NO.:116241356
OFFICIAL SYMBOL:ESX1
GENE ID:80712

Background

BACKGROUND:ESX1 Antibody: Homeobox proteins are transcription factors that contain a helix-turn-helix DNA binding domain termed the homeodomain. ESX1 is an X-linked homeobox gene primarily expressed in the placenta and testis and contains two functional domains: the homeodomain and the proline-rich domain. During embryogenesis, ESX1 is expressed in the extraembryonic tissues, including the endoderm of the visceral yolk sac, the ectoderm of the chorion and the labyrinthine trophoblast of the chorioallantoic placenta. ESX1 can act like a transcriptional repressor to the human oncogene K-ras and treatment of human cancer cells with an ESX1 protein fragment containing the homeodomain reduces the tumorgenicity of cells containing oncogenic K-ras mutations, suggesting ESX1 may be useful as a therapeutic treatment for these cancers.
REFERENCES: 1) Gehring WJ, Affolter M and Burglin T. Homeodomain proteins. Annu. Rev. Biochem.1994; 63:487-526.
2) Fohn LE and Behringer RR. ESX1L, a novel X chromosome-linked human homeobox gene expressed in the placenta and testis. Genomics2001; 74:105-8.
3) Li Y, Lemaire P and Behringer RR. ESX1, a novel X chromosome-linked homeobox gene expressed in mouse extraembryonic tissues and male germ cells. Dev. Biol.1997; 188:85-95.
4) Figueiredo AL, Salles MG, Albano RM, et al. Molecular and morphogenic analyses of expression of ESX1L in different stages of human placental development. J. Cell Mol. Med.2004; 8:545-50.

For Research Use Only