Estrogen Receptor beta Antibody [ERb455] Cat. No.: 33-129

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Horse, Human, Monkey, Mouse, Pig, Rat, Sheep
IMMUNOGEN: The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.
TESTED APPLICATIONS: Flow, IF, IHC, WB
APPLICATIONS: Flow Cytometry: 0.5-1ug/10e6 cells

IF: 1-2 ug/ml

WB: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Estrogen Receptor beta antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.


psi-iconProperties
PURIFICATION:Protein G purified monoclonal antibody
CLONALITY:Monoclonal
ISOTYPE:IgG2a
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:ESR2
ALTERNATE NAMES:Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2, ESR2, ESTRB, NR3A2
GENE ID:2100
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including alpha and beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER alpha and beta are differentially activated by various ligands. Ligand interaction triggers a cascade of events, including dissociation from heat shock proteins, recepter dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER alpha and beta may be regulated by distinct mechanisms even though they share many functional characteristics.

ANTIBODIES FOR RESEARCH USE ONLY.

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