ESRRB Antibody Cat. No.: 6813

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (89%), Rat: (89%)
IMMUNOGEN: ESRRB antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ESRRB.

The immunogen is located within amino acids 360 - 410 of ESRRB.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: ESRRB antibody can be used for detection of ESRRB by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide

psi-iconProperties
PURIFICATION:ESRRB Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ESRRB Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ESRRB antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ESRRB
ALTERNATE NAMES:ESRRB Antibody: ERR2, ERRb, ESRL2, NR3B2, DFNB35, ERRB2, Steroid hormone receptor ERR2, ERR beta-2, ERR-beta
ACCESSION NO.:NP_004443
PROTEIN GI NO.:238550159
GENE ID:2103
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ESRRB Antibody: The estrogen-related receptor beta (ESRRB) was initially identified in mouse embryonic carcinoma stem cells and was found to be expressed exclusively in trophoblast progenitor cells between days 6.5 and 7.5 post coitum. Recent studies have shown that ESRRB is part of an POU5F1/Oct4-centered protein interaction network in embryonic stem (ES) cells. It is thought to interact with other ES transcription factors such as NANOG within this network, activating POU5F1 transcription and thereby sustaining self-renewal and pluripotency in ES cells.
REFERENCES:1) Pettersson K, Svensson K, Mattsson R, et al. Expression of a novel member of estrogen response element-binding nuclear receptors is restricted to the early stages of chorion formation during mouse embryogenesis. Mech. Dev. 1996; 54:211-23.
2) van den Berg DL, Snoek T, Mullin NP, et al. An Oct4-centered protein interaction network in embryonic stem cells. Cell Stem Cell 2010; 6:369-81.
3) Zhang X, Zhang J, Wang T, et al. Esrrb activates Oct4 transcription and sustains self-renewal and pluripotency in embryonic stem cells. J. Biol. Chem. 2008; 283:35825-33.

ANTIBODIES FOR RESEARCH USE ONLY.

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