ESR1 Antibody Cat. No.: 7437

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Horse: (100%)
IMMUNOGEN: ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1.

The immunogen is located within amino acids 250 - 300 of ESR1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
SPECIFICITY: ESR1 antibody is human, mouse and rat reactive.
POSITIVE CONTROL:1) Cat. No. 1463 - Rat Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 65 kDa

Observed: 62 kDa

psi-iconProperties
PURIFICATION:ESR1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ESR1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ESR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:ESR1
ALTERNATE NAMES:ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER
ACCESSION NO.:NP_001116214
PROTEIN GI NO.:170295804
GENE ID:2099
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ESR1 Antibody: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
REFERENCES:1) Pakdel F, Reese JC, and Katzenellenbogen BS. Identification of charged residues in an N- terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol. Endocrinol. 1993; 7:1408-17.
2) Sheeler CQ, Singleton DW, and Khan SA. Mutation of serines 104, 106, and 118 inhibits dimerization of the human estrogen receptor in yeast. Endocr. Res. 2003; 29:237-55.
3) Rogatsky I, Trowbridge JM, and Garabedian MJ. Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex. J. Biol. Chem. 1999; 274:22296-302.

ANTIBODIES FOR RESEARCH USE ONLY.

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