Datasheet

ERp72 Antibody
CATALOG NUMBER: 8211

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (80%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:ERp72 antibody can be used for detection of ERp72 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 71 kDa

Observed: 75 kDa
SPECIFICITY:ERP72 antibody is human, mouse and rat reactive.
IMMUNOGEN:ERp72 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ERp72.

The immunogen is located within the last 50 amino acids of ERp72.
HOST SPECIES:Rabbit

Properties

PURIFICATION:ERp72 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ERp72 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ERp72 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Protein disulfide isomerase family A member 4, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ERp70, ERp-72
ACCESSION NO.:NP_004902
PROTEIN GI NO.:4758304
OFFICIAL SYMBOL:PDIA4
GENE ID:9601

Background

BACKGROUND:Protein disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (1,2). ER stress protein 72 (ERp72), also designated protein disulfide-isomerase A4, is involved in the catalysis of protein -S-S- bond rearrangement (3). It plays a role in the formation and isomerization of disulfide bonds (3,4).
REFERENCES: 1) Huppa JB and Ploegh HL. The eS-Sence of -SH in the ER. Cell 1998; 92:145-8.
2) Ellgaard L and Ruddock LW. The human protein disulphide isomerase family: substrate interactions and functional properties. EMBO Rep. 2005; 6:28-32.
3) Mazzarella RA, Srinivasan M, Haugejorden SM, et al. ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase. J. Biol. Chem. 1990; 265:1094-101.
4) Satoh M, Shimada A, Keino H, et al. Functional characterization of 3 thioredoxin homology domains of ERp72. Cell Stress Chaperones 2005; 10:278-84.

For Research Use Only