Datasheet

EPAC1 Antibody
CATALOG NUMBER: 6631

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:EPAC1 antibody can be used for detection of EPAC1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate
SPECIFICITY:At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.
IMMUNOGEN:EPAC1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EPAC1.

The immunogen is located within amino acids 60 - 110 of EPAC1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:EPAC1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:EPAC1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EPAC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:EPAC1 Antibody: EPAC, EPAC1, bcm910, HSU79275, CAMP-GEFI, CGEF1, EPAC, Rap guanine nucleotide exchange factor 3, Exchange factor directly activated by cAMP 1, EPAC 1
ACCESSION NO.:NP_001092001
PROTEIN GI NO.:148747859
OFFICIAL SYMBOL:RAPGEF3
GENE ID:10411

Background

BACKGROUND:EPAC1 Antibody: EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.
REFERENCES: 1) de Rooij J, Zwartkruis FJ, Verheijen MH, et al. Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP. Nature 1998; 396:474-7.
2) Bos JL. Epac: a new cAMP target and new avenues. Hum. Immunol. 2004; 65:282-90.
3) Holz GG, Kang G, Harbeck M, et al. Cell physiology of cAMP sensor Epac. J. Physiol. 2006; 577:5-15.

For Research Use Only