EndoG Antibody [7G1C10] Cat. No.: PM-4577

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Recombinant protein corresponding to amino acids 51 - 140 of human EndoG.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: EndoG monoclonal antibody can be used for detection of EndoG by Western blot at 2.5 - 5 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
 2) Cat. No. 95-103 - EndoG Recombinant Protein

psi-iconProperties
PURIFICATION:EndoG Monoclonal Antibody is Protein A purified.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:EndoG Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EndoG monoclonal antibody can be stored at -20˚C, stable for one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:ENDOG
ALTERNATE NAMES:EndoG Antibody [7G1C10] : Endo G
ACCESSION NO.:Q14249
PROTEIN GI NO.:24638471
GENE ID:2021
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:EndoG Monoclonal Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.
REFERENCES:1) Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9.
2) Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature 2001; 412:90-4
3) Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29.
4) Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9.

ANTIBODIES FOR RESEARCH USE ONLY.

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