Datasheet

EndoG Antibody
CATALOG NUMBER: 3035

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:EndoG antibody can be used for detection of EndoG by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1211 - HepG2 Cell Lysate
 2) Cat. No. 1212 - 3T3 Cell Lysate
 3) Cat. No. 1307 - Human Pancreas Tissue Lysate
 4) Cat. No. 11-101 - Human Pancreas Tissue Slide
PREDICTED MOLECULAR WEIGHT:35 kDa
IMMUNOGEN:EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

The immunogen is located within amino acids 40 - 90 of EndoG.
HOST SPECIES:Rabbit

Properties

PURIFICATION:EndoG Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:EndoG Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EndoG antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:EndoG Antibody: Endonuclease G, mitochondrial, Endo G
ACCESSION NO.:NP_004426
PROTEIN GI NO.:53759134
OFFICIAL SYMBOL:ENDOG
GENE ID:2021

Background

BACKGROUND:EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.
REFERENCES: 1) Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature. 2001;412(6842):95-9.
2) Parrish J, Li L, Klotz K, Ledwich D, Wang X, Xue D. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature. 2001;412(6842):90-4
3) Hengartner MO. Apoptosis. DNA destroyers. Nature. 2001;412(6842):27, 29.
4) Widlak P, Li LY, Wang X, Garrard WT. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J Biol Chem. 2001;276(51):48404-9.

For Research Use Only