Datasheet

ENC-2 Antibody
CATALOG NUMBER: 5695

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1205 - Jurkat Cell Lysate
 2) Cat. No. 17-005 - Jurkat Cell Slide
IMMUNOGEN:ENC-2 antibody was raised against a 14 amino acid synthetic peptide near the center of human ENC-2.

The immunogen is located within amino acids 230 - 280 of ENC-2.
HOST SPECIES:Chicken

Properties

PURIFICATION:ENC-2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:ENC-2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ENC-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:ENC-2 Antibody: ENC2, ENC-2, ENC2, Kelch-like protein 25, Ectoderm-neural cortex protein 2
ACCESSION NO.:AAV51405
PROTEIN GI NO.:55418570
OFFICIAL SYMBOL:KLHL25
GENE ID:64410

Background

BACKGROUND:ENC-2 Antibody: The ectoderm-neural cortex-2 (ENC-2) protein a member of the BTB/PDZ Kelch protein family displaying significant homology to ENC-1, an actin-binding protein involved in neural fate specification during embryogenesis and adipocyte maturation. The exact function of ENC-2 has yet to be determined, but it has been suggested to be involved in the differentiation of neuronal precursor cells into neurons and astrocytes.
REFERENCES: 1) Hernandez MC, Andres-Barquin PJ, Martinez S, et al. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J. Neurosci.1997; 17:3038-51.
2) Zhao L, Gregoire F and Sul HS. Transient induction of ENC-1, a Kelch-related actin-binding protein, is required for adipocyte differentiation. J. Biol. Chem.2000; 275:16845-50.
3) Ahn JI, Kim SY, Ko MJ, et al. Analysis of gene expression in mouse spinal cord-derived neural precursor cells during neuronal differentiation. Gen. and Infor.2009; 7:85-96.

For Research Use Only