Datasheet

EMX2 Antibody
CATALOG NUMBER: 4099

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (94%), Bovine: (94%)
TESTED APPLICATIONS:ELISA, IHC-P
APPLICATIONS:EMX2 antibody can be used for detection of EMX2 by immunohistochemistry at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 10-635 - Human Prostate Tissue Slide
PREDICTED MOLECULAR WEIGHT:28 kDa
SPECIFICITY:At least two isoforms of EMX2 are known to exist; this antibody will detect both isoforms. EMX2 antibody is predicted to not cross-react with EMX1.
IMMUNOGEN:EMX2 antibody was raised against an 16 amino acid synthetic peptide near the amino terminus of human EMX2.

The immunogen is located within the first 50 amino acids of EMX2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:EMX2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:EMX2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EMX2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:EMX2 Antibody: Homeobox protein EMX2, Empty spiracles homolog 2
ACCESSION NO.:Q04743
PROTEIN GI NO.:19862512
OFFICIAL SYMBOL:EMX2
GENE ID:2018

Background

BACKGROUND:EMX2 Antibody: EMX2, like its closely related homolog EMX1, is a homeobox transcription factor involved in specifying cell fates in the developing central nervous system and participates in the development of olfactory neurons. EMX2 is expressed in the dorsal telencephalon during development in a low rostral-lateral to high caudal-medial gradient and is proposed to pattern the neocortex into defined functional areas. It is also expressed in embryonic and adult olfactory neuroepithelia where it complexes with eukaryotic translation initiation factor 4E (eIF4E) and possibly regulates mRNA transport or translation. In the developing urogenital system, it is expressed in epithelial tissues and is negatively regulated by HOXA10.
REFERENCES: 1) Bishop KM, Garel S, Nakagawa Y, et al. EMX1 and Emx2 cooperate to regulate cortical size, lamination, neuronal differentiation, development of cortical efferents, and thalamocortical pathfinding. J. Comp. Neurol. 2003; 457:345-60.
2) Lichtneckert R, Nobs L, Reichert H. Empty spiracles is required for the development of olfactory projection neuron circuitry in Drosophila. Development 2008; 135:2415-24.
3) Gulisano M, et al. EMX2 and Emx2 show different patterns of expression during proliferation and differentiation of the developing cerebral cortex in the mouse. Eur. J. Neurosci. 1996; 8:1037-50.
4) Nedelec S, Foucher I, Brunet I, et al. Emx2 homeodomain transcription factor interacts with eukaryotic translation initiation factor 4E (eIF4E) in the axons of olfactory sensory neurons. Proc. Natl. Acad. Sci. USA 2004; 101:10815-20.

For Research Use Only