Datasheet

EMX1 Antibody
CATALOG NUMBER: 6625

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:EMX1 antibody can be used for detection of EMX1 by Western blot at 1 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
SPECIFICITY:At least two isoforms of EMX1 are known to exist; this antibody will detect the longer isoform. EMX1 antibody is predicted to not cross-react with other EMX family members.
IMMUNOGEN:EMX1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human EMX1.

The immunogen is located within amino acids 50 - 100 of EMX1.
HOST SPECIES:Chicken

Properties

PURIFICATION:EMX1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:EMX1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EMX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgY
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:EMX1 Antibody: Homeobox protein EMX1, Empty spiracles homolog 1
ACCESSION NO.:NP_004088
PROTEIN GI NO.:94536800
OFFICIAL SYMBOL:EMX1
GENE ID:2016

Background

BACKGROUND:EMX1 Antibody: EMX1 is a homeobox transcription factor involved in specifying cell fates in the developing central nervous system and it participates in the development of olfactory neurons. EMX1 is specifically expressed in the developing telencephalic cortex expression, where epxression is restricted to pyramidal neurons. EMX1 is a reliable marker of pyramidal neurons and pyramidal cell lineage. EMX1 has been shown to be one of the downstream target genes for Gli-Kruppel family member 3 (Gli3) transcription factor, which is a part of the Sonic hedgehog-Patched-Gli signaling pathway important in endocrine signaling.
REFERENCES: 1) Bishop KM, Garel S, Nakagawa Y, et al. Emx1 and Emx2 cooperate to regulate cortical size, lamination, neuronal differentiation, development of cortical efferents, and thalamocortical pathfinding. J. Comp. Neurol. 2003; 457:345-60.
2) Lichtneckert R, Nobs L, Reichert H. Empty spiracles is required for the development of olfactory projection neuron circuitry in Drosophila. Development 2008; 135:2415-24
3) Gulisano M, et al. Emx1 and Emx2 show different patterns of expression during proliferation and differentiation of the developing cerebral cortex in the mouse. Eur. J. Neurosci. 1996; 8:1037-50.
4) Chan CH, Godinho LN, Thomidou D, et al. 2001. Emx1 is a marker for pyramidal neurons of the cerebral cortex. Cereb. Cortex 11:1191-8.

For Research Use Only