Datasheet

EIG121 Antibody
CATALOG NUMBER: 6443

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Mouse: (100%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:EIG121 antibody can be used for detection of EIG121 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1219 - MCF7 Cell Lysate
 2) Cat. No. 17-012 - MCF7 Cell Slide
SPECIFICITY:At least four isoforms of EIG121 known to exist. EIG121 antibody is predicted to not cross-react with other UPF0577 protein family members.
IMMUNOGEN:EIG121 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human EIG121.

The immunogen is located within the last 50 amino acids of EIG121.
HOST SPECIES:Rabbit

Properties

PURIFICATION:EIG121 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:EIG121 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EIG121 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:EIG121 Antibody: EIG, EIG1
ACCESSION NO.:NP_065826
PROTEIN GI NO.:38569482
OFFICIAL SYMBOL:EGI
GENE ID:1957

Background

BACKGROUND:EIG121 Antibody: EIG121 (Estrogen-induced gene 121 protein) is thought to play a role as a marker of hyperestrogenic state and estrogen-related type I endometrial carcinoma. It belongs to the UPF0577 family. It is a 1013 amino acid single-pass transmembrane protein that, though expressed in normal endometrium, is overexpressed in endometriod tumors. As an evolutionarily conserved gene, EIG121 is also expressed during early xenopus development, showing maximum expression at the gastrula stage.
REFERENCES: 1) Deng L, Broaddus RR, McCampbell A, et al. Identification of a novel estrogen-regulated gene, EIG121, induced by hormone replacement therapy and differentially expressed in type I and type II endometrial cancer. Clin. Cancer Res. 2005; 11:8258-64.
2) Deng L, Feng J, Broaddus RR. The novel estrogen-induced gene EIG121 regulates autophagy and promotes cell survival under stress. Cell Death Dis. 2010; 1:e32.
3) Araki T, Kusakabe M and Nishida E. Expression of estrogen induced gene 121-like (EIG121L) during early Xenopus development. Gene Expr. Patterns 2007; 7:666-71.

For Research Use Only