EIG121 Antibody Cat. No.: 6443

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (100%)
IMMUNOGEN: EIG121 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human EIG121.

The immunogen is located within the last 50 amino acids of EIG121.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: EIG121 antibody can be used for detection of EIG121 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least four isoforms of EIG121 known to exist. EIG121 antibody is predicted to not cross-react with other UPF0577 protein family members.
POSITIVE CONTROL:1) Cat. No. 1219 - MCF7 Cell Lysate
 2) Cat. No. 17-012 - MCF7 Cell Slide

psi-iconProperties
PURIFICATION:EIG121 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:EIG121 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EIG121 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:EGI
ALTERNATE NAMES:EIG121 Antibody: EIG, EIG1
ACCESSION NO.:NP_065826
PROTEIN GI NO.:38569482
GENE ID:1957
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:EIG121 Antibody: EIG121 (Estrogen-induced gene 121 protein) is thought to play a role as a marker of hyperestrogenic state and estrogen-related type I endometrial carcinoma. It belongs to the UPF0577 family. It is a 1013 amino acid single-pass transmembrane protein that, though expressed in normal endometrium, is overexpressed in endometriod tumors. As an evolutionarily conserved gene, EIG121 is also expressed during early xenopus development, showing maximum expression at the gastrula stage.
REFERENCES:1) Deng L, Broaddus RR, McCampbell A, et al. Identification of a novel estrogen-regulated gene, EIG121, induced by hormone replacement therapy and differentially expressed in type I and type II endometrial cancer. Clin. Cancer Res. 2005; 11:8258-64.
2) Deng L, Feng J, Broaddus RR. The novel estrogen-induced gene EIG121 regulates autophagy and promotes cell survival under stress. Cell Death Dis. 2010; 1:e32.
3) Araki T, Kusakabe M and Nishida E. Expression of estrogen induced gene 121-like (EIG121L) during early Xenopus development. Gene Expr. Patterns 2007; 7:666-71.

ANTIBODIES FOR RESEARCH USE ONLY.

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