Datasheet

EFHD1 Antibody
CATALOG NUMBER: 5655

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IF, WB
APPLICATIONS:EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunoflourescence starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
IMMUNOGEN:EFHD1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1.

The immunogen is located within the first 50 amino acids of EFHD1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:EFHD1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:EFHD1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EFHD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:EFHD1 Antibody: SWS2, MST133, PP3051, MSTP133, SWS2, EF-hand domain-containing protein D1, EF-hand domain-containing protein 1
ACCESSION NO.:NP_079478
PROTEIN GI NO.:20149496
OFFICIAL SYMBOL:EFHD1
GENE ID:80303

Background

BACKGROUND:EFHD1 Antibody: EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.
REFERENCES: 1) Tominaga M and Tomooka Y. Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53-/- mouse. Biochem. Biophys. Res. Comm.2002; 297:473-9.

For Research Use Only