EFCAB4B Antibody Cat. No.: 6045

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: EFCAB4B antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human EFCAB4B.

The immunogen is located within amino acids 640 - 690 of EFCAB4B.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: EFCAB4B antibody can be used for detection of EFCAB4B by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1405 - Mouse Kidney Tissue Lysate
 2) Cat. No. 10-401 - Human Kidney Tissue Slide

psi-iconProperties
PURIFICATION:EFCAB4B Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:EFCAB4B Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EFCAB4B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:EFCAB4B
ALTERNATE NAMES:EFCAB4B Antibody: CRACR2A, CRACR2A, EF-hand calcium-binding domain-containing protein 4B, Calcium release-activated calcium channel regulator 2A, CRAC channel regulator 2A
ACCESSION NO.:NP_001138430
PROTEIN GI NO.:14249254
GENE ID:84766
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:EFCAB4B Antibody: EFCAB4B, also known as Calcium release-activated calcium channel regulator 2A, is a novel Ca2+-binding EF-hand protein that is thought to play a key role in store-operated Ca2+ entry in T-cells by regulating CRAC channel activation. EFCAB4B acts as a cytoplasmic calcium-sensor that forms a complex with ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration. A closely related protein, EFCAB4A, is likely to play a similar role as EFCAB4B, but the detailed function of EFCAB4A is still under investigation.
REFERENCES:1) Srikanth S, Jung HJ, Kim KD, et al. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell. Biol.2010; 12:436-46.
2) Srikanth S, Jung HJ, Ribalet B, et al. The intracellular loop of Orai1 plays a central role in fast inactivation of Ca2+ release-activated Ca2+ channels. J. Biol. Chem.2010; 285:5066-75.
3) Maruyama K, Mikawa T, and Ebashi S. Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. J. Biochem.1984; 95:511-9.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.