Datasheet

EFCAB4A Antibody
CATALOG NUMBER: 6043

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:EFCAB4A antibody can be used for detection of EFCAB4A by Western blot at 1 - 2 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1302 - Human Lung Tissue Lysate
IMMUNOGEN:EFCAB4A antibody was raised against a 15 amino acid synthetic peptide near the center of human EFCAB4A.

The immunogen is located within amino acids 290 - 340 of EFCAB4A.
HOST SPECIES:Rabbit

Properties

PURIFICATION:EFCAB4A Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:EFCAB4A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EFCAB4A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:EFCAB4A Antibody: CRACR2B, CRACR2B, EF-hand calcium-binding domain-containing protein 4A, Calcium release-activated calcium channel regulator 2B, CRAC channel regulator 2B
ACCESSION NO.:NP_775855
PROTEIN GI NO.:150170653
OFFICIAL SYMBOL:EFCAB4A
GENE ID:283229

Background

BACKGROUND:EFCAB4A Antibody: EFCAB4A, also known as Calcium release-activated calcium channel regulator 2B, is a novel Ca2+-binding EF-hand protein that is thought to play a key role in store-operated Ca2+ entry in T-cells by regulating CRAC channel activation, but the detailed function is still under investigation. It is likely to play a similar role as the related protein EFCAB4B, which acts as a cytoplasmic calcium-sensor that forms a complex with ORAI1 and STIM1 at the junctional regions between the plasma membrane and the endoplasmic reticulum upon low Ca2+ concentration.
REFERENCES: 1) Srikanth S, Jung HJ, Kim KD, et al. A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that stabilizes CRAC channels in T cells. Nat. Cell. Biol.2010; 12:436-46.
2) Srikanth S, Jung HJ, Ribalet B, et al. The intracellular loop of Orai1 plays a central role in fast inactivation of Ca2+ release-activated Ca2+ channels. J. Biol. Chem.2010; 285:5066-75.
3) Maruyama K, Mikawa T, and Ebashi S. Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. J. Biochem.1984; 95:511-9.

For Research Use Only