EDA1 Antibody Cat. No.: 8045

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (89%)
IMMUNOGEN: EDA1 antibody was raised against an 19 amino acid peptide near the center of human EDA1.

The immunogen is located within amino acids 130 - 180 of EDA1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: EDA1 antibody can be used for detection of EDA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: EDA1 antibody is human, mouse and rat reactive. Multiple isoforms of EDA1 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1405 - Mouse Kidney Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 16, 43 kDa

Observed: 39 kDa

psi-iconProperties
PURIFICATION:EDA1 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:EDA1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EDA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:EDA
ALTERNATE NAMES:Ectodysplasin A, Ectodermal dysplasia protein, ED1, ED1-A1, ED1-A2, EDA1, EDA2, EDA protein, HED, ODT1, STHAGX1, XHED, XLHED
ACCESSION NO.:NP_001390
PROTEIN GI NO.:4503449
GENE ID:1896
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Ectodysplasin A (EDA1) is a member of the TNF-related ligand family involved in the early epithelial-mesenchymal interaction that regulates ectodermal appendage formation (1). It is a trimeric type II membrane protein that co-localizes with cytoskeletal structures at the lateral and apical surfaces of cells and can be expressed as eight alternatively spliced isoforms in hair follicles and in the epidermis of adult skin (2,3). EDAs are required during development, and loss or mutation of EDA1 results in a group of developmental disorders identified as ectodermal dysplasia type 1 (4,5).
REFERENCES:1) Kere J, Srivastava AK, Montonen O. X-linked anhidrotic (hypohidrotic) ectodermal dysplasia is caused by mutation in a novel transmembrane protein. Nat. Genet. 1996; 13:409-16.
2) Vincent MC, Biancalana V, Ginisty D, et al. Mutational spectrum of the ED1 gene in X-linked hypohidrotic ectodermal dysplasia. Eur. J. Hum. Genet. 2001; 9:355-63.
3) Ohashi M, Moriya C, Tanahashi K, et al. A new EDA gene mutation in a family of X-linked hypohidrotic ectodermal dysplasia. J. Dermatol. Sci. 2014; 74:175-7.
4) BayƩs M, Hartung AJ, Ezer S, et al. The anhidrotic ectodermal dysplasia gene (EDA) undergoes alternative splicing and encodes ectodysplasin-A with deletion mutations in collagenous repeats. Hum. Mol. Genet. 1998; 7:1661-9.

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