Specifications
| HOST SPECIES: | Goat |
| SPECIES REACTIVITY: |
Human |
| IMMUNOGEN: |
The immunogen for this antibody is: C-NNGKQSLSAEKVL |
| TESTED APPLICATIONS: |
ELISA, IF, IHC, WB |
| APPLICATIONS: |
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Lung and Human Umbilical Cord lysates (calculated MW of 21.3kDa according to NP_001071161.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ref PMID: 18556573). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Vessel. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Human: Matsuo K et al, J Biol Chem. 2015 Feb 6;290(6):3784. PMID: 25533470.Immunofluorescence: This product has been successfully used in IF on Human: Matsuo K et al, J Biol Chem. 2015 Feb 6;290(6):3784. PMID: 25533470. |
| PREDICTED MOLECULAR WEIGHT: |
Approx 50kDa |
Properties
| PURIFICATION: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| CLONALITY: | Polyclonal |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| CONCENTRATION: | 500 ug/mL |
| STORAGE CONDITIONS: | Aliquot and store at -20˚C. Minimize freezing and thawing. |
Additional Info
| OFFICIAL SYMBOL: | ECSM2 |
| ALTERNATE NAMES: | endothelial cell-specific marker 2, endothelial cell-specific molecule 2, hypothetical protein LOC641700 |
| ACCESSION NO.: | NP_001071161.1 |
| PROTEIN GI NO.: | 118130809 |
| GENE ID: | 641700 |
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