Ebolavirus Glycoprotein Recombinant Protein Cat. No.: 97-050

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psi-iconSpecifications
SPECIES:Ebolavirus
SOURCE SPECIES:HEK293 cells
SEQUENCE:Ile 33 - Arg 324
FUSION TAG:His Tag
TESTED APPLICATIONS:WB
APPLICATIONS:This recombinant protein can be used for WB. For research use only.
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:34.3 kDa
PHYSICAL STATE:Lyophilized
BUFFER:PBS, pH7.4
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:Ebolavirus Glycoprotein
ACCESSION NO.:B8XCN1
OFFICIAL SYMBOL:Glycoprotein / GP (virus)
GENE ID:9487265
psi-iconBackground and References
BACKGROUND:EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
REFERENCES:1) Saeed M.F., et al., 2010, PLoS Pathog. 6:0-0.
2) Marzi A., et al., 2006, J. Virol. 80:6305-6317.
3) Chandran K., et al., 2005, Science 308:1643-1645.

FOR RESEARCH USE ONLY.

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