EBI3 Antibody Cat. No.: 4967

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (46.7%), Rat: (46.7%)
IMMUNOGEN: Anti-EBI-3 antibody (4967) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of mature human EBI-3.

The immunogen is located within the last 50 amino acids of EBI3.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 2 μg/mL; IF: 20 μg/mL ; IHC: 2.5 μg/mL;.

Antibody validated: Western Blot, Immunofluorescence, and Immunohistochemistry in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1315 - Human Tonsil Tissue Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 25kD

Observed: 30 kD (25kD + 2 N-Glycosylations)
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 2) shows that anti-EBI-3 antibody (4967) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 4967 antigenic peptide.

 

ISOFORMS: Human EBI-3 has only 1 isoform (229aa, 25kD). Mouse EBI-3 has one isoform (228aa, 25kD) and rat EBI-3 also has one isoform (228aa, 25kD). 4967 can detect human EBI-3 but cannot detect mouse and rat EBI-3.

psi-iconProperties
PURIFICATION:EBI3 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:EBI3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:EBI3
ALTERNATE NAMES:EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta
ACCESSION NO.:NP_005746
PROTEIN GI NO.:14577917
GENE ID:10148
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.
REFERENCES:1) Pfanz et al. Immunity2002; 16:779-90.
2) Devergne et al. Proc. Natl. Acad. Sci. USA1997; 94:12041-6.
3) Niedbala  et al. Eur. J. Immunol.2007; 3021-29.
4) Pfanz et al. J. Immunol.2004; 172:2225-31.

ANTIBODIES FOR RESEARCH USE ONLY.

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