EAT2 (Phospho Tyr127) Antibody Cat. No.: 46-835

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human
IMMUNOGEN: EAT2 Phospho Y127 antibody was raised against a 12 amino acid synthetic peptide near the internal region of EAT2 Phospho Y127.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1μg/ml.

psi-iconProperties
PURIFICATION:EAT2 Phospho Y127 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:EAT2 pH ospH o Y127 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:SH2D1B
ALTERNATE NAMES:SH2D1B, SH2 domain containing 1B, EAT2, SH2 domain-containing molecule EAT2
ACCESSION NO.:NP_444512.2
PROTEIN GI NO.:54792745
GENE ID:117157
psi-iconBackground and References
REFERENCES:1) Morra, M.; Lu, J.; Poy, F.; Martin, M.; Sayos, J.; Calpe, S.; Gullo, C.; Howie, D.; Rietdijk, S.; Thompson, A.; Coyle, A. J.; Denny, C.; Yaffe, M. B.; Engel, P.; Eck, M. J.; Terhorst, C. Structural basis for the interaction of the free SH2 domain EAT-2 with SLAM receptors in hematopoietic cells. EMBO J. 20: 5840-5852, 2001

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