Specifications
HOST SPECIES: | Goat |
SPECIES REACTIVITY: |
Human |
IMMUNOGEN: |
The immunogen for this antibody is: C-NSNSDpYVDVLP |
TESTED APPLICATIONS: |
ELISA, WB |
APPLICATIONS: |
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1ug/ml. |
Properties
PURIFICATION: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Subsequently passed through a column with the non-phosphorylated version of the immunizing peptide. |
CLONALITY: | Polyclonal |
CONJUGATE: | Unconjugated |
PHYSICAL STATE: | Liquid |
BUFFER: | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
CONCENTRATION: | 500 ug/mL |
STORAGE CONDITIONS: | Aliquot and store at -20˚C. Minimize freezing and thawing. |
Additional Info
OFFICIAL SYMBOL: | SH2D1B |
ALTERNATE NAMES: | SH2D1B, SH2 domain containing 1B, EAT2, SH2 domain-containing molecule EAT2 |
ACCESSION NO.: | NP_444512.2 |
PROTEIN GI NO.: | 54792745 |
GENE ID: | 117157 |
Background and References
REFERENCES: | 1) Morra, M.; Lu, J.; Poy, F.; Martin, M.; Sayos, J.; Calpe, S.; Gullo, C.; Howie, D.; Rietdijk, S.; Thompson, A.; Coyle, A. J.; Denny, C.; Yaffe, M. B.; Engel, P.; Eck, M. J.; Terhorst, C. Structural basis for the interaction of the free SH2 domain EAT-2 with SLAM receptors in hematopoietic cells. EMBO J. 20: 5840-5852, 2001 |
ANTIBODIES FOR RESEARCH USE ONLY.
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