Specifications
| HOST SPECIES: | Goat |
| SPECIES REACTIVITY: |
Human |
| IMMUNOGEN: |
EAT2 Phospho Y127 antibody was raised against a 12 amino acid synthetic peptide near the internal region of EAT2 Phospho Y127. |
| TESTED APPLICATIONS: |
ELISA, WB |
| APPLICATIONS: |
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 14kDa band observed in lysates of cell line A431 (calculated MW of 16.6kDa according to NP_002643.1). Recommended concentration: 0.3-1μg/ml. |
Properties
| PURIFICATION: | EAT2 Phospho Y127 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| CLONALITY: | Polyclonal |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | EAT2 pH ospH o Y127 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. |
| CONCENTRATION: | 500 ug/mL |
| STORAGE CONDITIONS: | Aliquot and store at -20˚C. Minimize freezing and thawing. |
Additional Info
| OFFICIAL SYMBOL: | SH2D1B |
| ALTERNATE NAMES: | SH2D1B, SH2 domain containing 1B, EAT2, SH2 domain-containing molecule EAT2 |
| ACCESSION NO.: | NP_444512.2 |
| PROTEIN GI NO.: | 54792745 |
| GENE ID: | 117157 |
Background and References
| REFERENCES: | 1) Morra, M.; Lu, J.; Poy, F.; Martin, M.; Sayos, J.; Calpe, S.; Gullo, C.; Howie, D.; Rietdijk, S.; Thompson, A.; Coyle, A. J.; Denny, C.; Yaffe, M. B.; Engel, P.; Eck, M. J.; Terhorst, C. Structural basis for the interaction of the free SH2 domain EAT-2 with SLAM receptors in hematopoietic cells. EMBO J. 20: 5840-5852, 2001 |
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