E2F5 Antibody Cat. No.: 63-739

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse, Rat, Xenopus
IMMUNOGEN: This E2F5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the N-terminal region of human E2F5.
TESTED APPLICATIONS: Flow, IHC-P, WB
APPLICATIONS: For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
PREDICTED MOLECULAR WEIGHT: 38 kDa

psi-iconProperties
PURIFICATION:This antibody is purified through a protein A column, followed by peptide affinity purification.
CLONALITY:Polyclonal
ISOTYPE:Rabbit Ig
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:E2F5
ALTERNATE NAMES:Transcription factor E2F5, E2F-5, E2F5
ACCESSION NO.:Q15329
PROTEIN GI NO.:2494230
GENE ID:1875
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
REFERENCES:1) Itoh,A., et.al., Cell. Mol. Biol. Res. 41 (3), 147-154 (1995)

ANTIBODIES FOR RESEARCH USE ONLY.

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