E2F3 Antibody Cat. No.: 7929

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: E2F3 antibody was raised against a 15 amino acid peptide near the center of human E2F3.

The immunogen is located within amino acids 150 - 200 of E2F3.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: E2F3 antibody can be used for detection of E2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: E2F3 antibody is human and mouse reactive. At least three isoforms of E2F3 are known to exist; this antibody will detect only the largest isoform. This antibody is predicted to not cross-react with other members of the E2F transcription factor family.
POSITIVE CONTROL:1) Cat. No. 1369 - Human Lymph node Tissue Lysate
 2) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 51 kDa

Observed: 56 kDa

psi-iconProperties
PURIFICATION:E2F3 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:E2F3 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:E2F3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:E2F3
ALTERNATE NAMES:E2F transcription factor 3, E2F-3
ACCESSION NO.:NP_001940
PROTEIN GI NO.:4503433
GENE ID:1871
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The E2F transcription factor 3 (E2F3) is a member of a small family of transcription factors that function through binding of DP interaction partner proteins. E2F3 recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle (1). Like the related E2F1 and E2F2, E2F3 is essential for cellular proliferation and progression through the cell cycle (2). Altered copy number and activity of this gene have been observed in a number of human cancers (3).
REFERENCES:1) Lees JA, Saito M, Vidal M, et al. The retinoblastoma protein binds to a family of E2F transcription factors. Mol. Cell. Biol. 1993; 13:7813-25.
2) Wu L, Timmers C, Maiti B, et al. The E2F1-3 transcription factors are essential for cellular proliferation. Nature 2001; 414:457-62.
3) DeGregori J. The genetics of the E2F family of transcription factors: shared functions and unique roles. Biochim. Biophys. Acta. 2002; 1602:131-50.

ANTIBODIES FOR RESEARCH USE ONLY.

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