| HOST SPECIES: | Mouse |
| SPECIES REACTIVITY: | E. coli O157 |
| IMMUNOGEN: | A crude sonicate of E. coli O157 was used as the immunogen for the E. coli O157 antibody. |
| TESTED APPLICATIONS: | ELISA, IF |
| APPLICATIONS: | ELISA: 1-5 ug/ml; order BSA free formulation for coating IF: 1-2 ug/ml Optimal dilution of the E. coli O157 antibody should be determined by the researcher. |
Specifications| PURIFICATION: | PEG precipitation followed by dialysis in 1X PBS |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgM, kappa |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| CONCENTRATION: | 0.2 mg/mL |
| STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
| BACKGROUND: | This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli. |
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