Datasheet

DYRK2 Antibody
CATALOG NUMBER: 6257

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (89%), Mouse: (89%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:DYRK2 antibody can be used for detection of DYRK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide
SPECIFICITY:Two isoforms of DYRK2 are known to exist; this antibody will recognize both isoforms. DYRK2 antibody will not cross-react with other DYRK family members.
IMMUNOGEN:DYRK2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DYRK2.

The immunogen is located within amino acids 520 - 570 of DYRK2.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DYRK2 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DYRK2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DYRK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DYRK2 Antibody: Dual specificity tyrosine-phosphorylation-regulated kinase 2
ACCESSION NO.:NP_006473
PROTEIN GI NO.:4503427
OFFICIAL SYMBOL:DYRK2
GENE ID:8445

Background

BACKGROUND:DYRK2 Antibody: DYRK2 is a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family that is thought to be involved in cellular proliferation and apoptosis. Under normal conditions, nuclear but not cytoplasmic DYRK2 is ubiquitinated by MDM2, leading to its constitutive degradation. However, upon exposure to genotoxic stress, ATM phosphorylates DYRK2, leading to its dissociation from MDM2 and its phosphorylation of p53, thereby inducing apoptosis. Recent evidence also suggests that DYRK2 may serve as a scaffold that facilitates assembly of an E3 ubiquitin ligase.
REFERENCES: 1) Becker W, Weber Y, Wetzel K, et al. Sequence characteristics, subcellular localization, and substrate specificity of DRYK-related kinases, a novel family of dual specificity protein kinases. J. Biol. Chem. 1998; 273:25893-902.
2) Yoshida K. Role for DYRK family kinases on regulation of apoptosis. Biochem. Pharmacol. 2008; 76:1389-94.
3) Taira N, Yamamoto H, Yamaguchi T, et al. ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage J. Biol. Chem. 2010; 285:4909-19.

For Research Use Only