DYRK1A Antibody Cat. No.: 6255

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: DYRK1A antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human DYRK1A.

The immunogen is located within the last 50 amino acids of DYRK1A.
TESTED APPLICATIONS: ELISA, ICC, WB
APPLICATIONS: DYRK1A antibody can be used for detection of DYRK1A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: Multiple isoforms of DYRK1A are known to exist. DYRK1A antibody will not cross-react with other DYRK family members
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
 3) Cat. No. 1204 – K562 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 84 kDa

Observed: 90 kDa

psi-iconProperties
PURIFICATION:DYRK1A Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DYRK1A Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DYRK1A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:DYRK1A
ALTERNATE NAMES:DYRK1A Antibody: MNB, DYRK, HP86, MNBH, MRD7, DYRK1, MNB, Dual specificity tyrosine-phosphorylation-regulated kinase 1A, Dual specificity YAK1-related kinase
ACCESSION NO.:NP_001387
PROTEIN GI NO.:18765758
GENE ID:1859
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DYRK1A Antibody: DYRK1A is a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family and is thought to be involved in neuronal differentiation, neurodegenerative diseases, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. DYRK1A phosphorylates several transcription factors such as p53, leading to inhibition of embryonic neuronal cell proliferation. DYRK1A associates with multiple proteins, including SPRED1 and SPRED2; this association appears to inhibit the phosphorylation activity of DYRK1A. Recent evidence also suggests that DYRK1A may be involved in the replication of HIV-1.
REFERENCES:1) Park J, Oh Y, and Chung KC. Two key genes closely implicated with the neuropathological characteristics in Down Syndrome: DYRK1A and RCAN1. BMB Reports 2009; 42:6-15.
2) Park J, Oh Y, You L, et al. Dyrk1A phosphorylates p53 and inhibits proliferation of embryonic neuronal cells. J. Biol. Chem. 2010; 285:31895-906
3) Li D, Jackson RA, Yusoff P, et al. Direct association of Sprouty-related protein with and EVH1 domain (SPRED) 1 or SPRED2 with DYRK1A modifies substrate/kinase interactions. J. Biol. Chem. 2010; 285:35374-85.

ANTIBODIES FOR RESEARCH USE ONLY.

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