| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human, Mouse |
| HOMOLOGY: | Predicted species reactivity based on immunogen sequence: Chicken: (83%) |
| IMMUNOGEN: | DPF3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DPF3. The immunogen is located within amino acids 220 - 270 of DPF3. |
| TESTED APPLICATIONS: | ELISA, IF, IHC-P, WB |
| APPLICATIONS: | DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
| SPECIFICITY: | DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist. |
| POSITIVE CONTROL: | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
| 2) Cat. No. 10-301 - Human Brain Tissue Slide | |
| PREDICTED MOLECULAR WEIGHT: | Predicted: 39 kDa Observed: 50 kDa |
Specifications| PURIFICATION: | DPF3 antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | DPF3 antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | DPF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| OFFICIAL SYMBOL: | DPF3 |
| ALTERNATE NAMES: | DPF3 Antibody: CERD4, BAF45C, CERD4, Zinc finger protein DPF3, BRG1-associated factor 45C |
| ACCESSION NO.: | NP_036206 |
| PROTEIN GI NO.: | 148762958 |
| GENE ID: | 8110 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
| BACKGROUND: | The DPF3 protein, also known as Cerd4, is a member of the d4 gene family of transcription modulators that also includes DPF1/Neud4 and DPF2/Requiem (1). DPF3 has been shown to be a epigenetic key factor for heart and muscle development and can bind to methylated and acetylated lysine residues of histone 3 and 4, suggesting that DPF3 may play a role in recruiting chromatin remodeling complexes to acetylated histones (2). Two isoforms of DPF3, DPF3a and DPF3b, are required as transcriptional co-activators in SWI/SNF complex-dependent activation of the NF-kappaB RelA/p50 heterodimer (3). |
| REFERENCES: | 1) Ninkina NN, Mertsalov IB, Kulikova DA, et al. Cerd4, third member of the d4 gene family: expression and organization of genomic locus. Mamm. Genome 2001; 12:862-6. | 2) Lange M, Kaynak B, Forster UB. Regulation of muscle development by DBF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex. Genes Dev. 2008; 22:2370-84. | 3) Ishizaka A, Mizutani T, Kobayashi K, et al. Double plant homeodomain (PHD) finger proteins DPF3a and -3b are required as transcriptional co-activators in SWI/SNF complex-dependent activation of NF-kappaB RelA/p50 heterodimer. J. Biol. Chem. 2012; 287:11924-33. |
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