Datasheet

DPF3 Antibody
CATALOG NUMBER: 7559

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Chicken: (83%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 39 kDa

Observed: 50 kDa
SPECIFICITY:DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.
IMMUNOGEN:DPF3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DPF3.

The immunogen is located within amino acids 220 - 270 of DPF3.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DPF3 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DPF3 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DPF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DPF3 Antibody: CERD4, BAF45C, CERD4, Zinc finger protein DPF3, BRG1-associated factor 45C
ACCESSION NO.:NP_036206
PROTEIN GI NO.:148762958
OFFICIAL SYMBOL:DPF3
GENE ID:8110

Background

BACKGROUND:The DPF3 protein, also known as Cerd4, is a member of the d4 gene family of transcription modulators that also includes DPF1/Neud4 and DPF2/Requiem (1). DPF3 has been shown to be a epigenetic key factor for heart and muscle development and can bind to methylated and acetylated lysine residues of histone 3 and 4, suggesting that DPF3 may play a role in recruiting chromatin remodeling complexes to acetylated histones (2). Two isoforms of DPF3, DPF3a and DPF3b, are required as transcriptional co-activators in SWI/SNF complex-dependent activation of the NF-kappaB RelA/p50 heterodimer (3).
REFERENCES: 1) Ninkina NN, Mertsalov IB, Kulikova DA, et al. Cerd4, third member of the d4 gene family: expression and organization of genomic locus. Mamm. Genome 2001; 12:862-6.
2) Lange M, Kaynak B, Forster UB. Regulation of muscle development by DBF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex. Genes Dev. 2008; 22:2370-84.
3) Ishizaka A, Mizutani T, Kobayashi K, et al. Double plant homeodomain (PHD) finger proteins DPF3a and -3b are required as transcriptional co-activators in SWI/SNF complex-dependent activation of NF-kappaB RelA/p50 heterodimer. J. Biol. Chem. 2012; 287:11924-33.

For Research Use Only