DOCK8 Antibody Cat. No.: 7847

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: DOCK8 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human DOCK8.

The immunogen is located within the last 50 amino acids of DOCK8.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist.
POSITIVE CONTROL:1) Cat. No. 1287 - EL4 Cell Lysate
 2) Cat. No. 10-901 - Human Spleen Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 166, 220, 231 kDa

Observed: 220, 230 kDa

psi-iconProperties
PURIFICATION:DOCK8 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DOCK8 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DOCK8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:DOCK8
ALTERNATE NAMES:DOCK8 Antibody: MRD2, ZIR8, HEL-205, Dedicator of cytokinesis protein 8
ACCESSION NO.:NP_982272
PROTEIN GI NO.:238231392
GENE ID:81704
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).
REFERENCES:1) Ruusala A and Aspenstrom P. Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology. FEBS Lett. 2004; 572:159-66.
2) Randall KL, Lambe T, Goodnow CC, et al. The essential role of DOCK8 in humoral immunity. Dis. Markers 2010; 29:141-50.
3) Engelhardt KR, McGhee S, Sinkler S, et al. Large deletions and point mutations involving the dedicator of cytokinesis 8 (DOCK8) in the autosomal recessive form of hyper-IgE syndrome. J. Allergy Clin. Immunol. 2009; 124:1289-302.

ANTIBODIES FOR RESEARCH USE ONLY.

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