DNM1L Antibody Cat. No.: 7603

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (100%), Bovine: (100%)
IMMUNOGEN: DNM1L antibody was raised against a 19 amino acid peptide near the center of human DNM1L.

The immunogen is located within amino acids 530 - 580 of DNM1L.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: DNM1L antibody can be used for detection of DNM1L by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: DNM1L antibody is human and mouse reactive. At least four isoforms of DNM1L are known to exist; this antibody will detect the two longest isoforms.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 17-001 - HeLa Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 81 kDa

Observed: 80 kDa

psi-iconProperties
PURIFICATION:DNM1L antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DNM1L antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DNM1L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:DNM1L
ALTERNATE NAMES:DNM1L Antibody: DLP1, DRP1, DVLP, EMPF, DYMPLE, HDYNIV, DLP1, Dynamin-1-like protein, Dynamin family member proline-rich carboxyl-terminal domain less
ACCESSION NO.:NP_036192
PROTEIN GI NO.:171460914
GENE ID:10059
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The Dynamin-1-like protein (DNM1L) is a member of the dynamin superfamily of GTPases (1). DNM1L mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis (2). Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF) (3).
REFERENCES:1) Shin HW, Shinotsuka C, Torii S, et al. Identification and subcellular localization of a novel mammalian dynamin-related protein homologous to yeast Vps1p and Dnm1p. J. Biochem. 1997; 122:525-30.
2) Frank S, Gaume B, Bergmann-Leitner ES, et al. The role of dynamin-related protein1, a mediator of mitochondrial fission, in apoptosis. Dev. Cell 2001; 1:515-25.
3) Reddy PH, Reddy TP, Manczak M, et al. Dynamin-related protein 1 and mitochondrial fragmentation in neurodegenerative diseases. Brain Res. Rev. 2011; 67:103-18.

ANTIBODIES FOR RESEARCH USE ONLY.

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