Datasheet

DNAL1 Antibody
CATALOG NUMBER: 4733

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, ICC, IF, WB
APPLICATIONS:DNAL1 antibody can be used for detection of DNAL1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
 2) Cat. No. 17-201 - 3T3/BALB Cell Slide
IMMUNOGEN:DNAL1 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human DNAL1.

The immunogen is located within the last 50 amino acids of DNAL1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DNAL1 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DNAL1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DNAL1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DNAL1 Antibody: CILD16, C14orf168, Dynein light chain 1, axonemal
ACCESSION NO.:NP_113615
PROTEIN GI NO.:164607156
OFFICIAL SYMBOL:DNAL1
GENE ID:83544

Background

BACKGROUND:DNAL1 Antibody: DNAL1 was identified as a potential candidate gene for primary ciliary dyskinesia (PCD), a genetically heterologous disorder characterized by chronic infections of the upper and lower airways that often leads to permanent lung damage, randomization of left/right body symmetry, and reduced fertility. DNAL1 is reported to be expressed solely in tissues carrying motile cilia for flagella and interacts with DNAH5, a protein that when mutated has been shown to result in PCD. It has been suggested that DNAL1 serves a regulatory function for DNAH5 activity in outer dynein arms of sperm flagella, respiratory cilia, and ependymal cilia. DNAL1 has also been recently identified as an HIV dependency factor (HDF), suggesting that DNAL1 may be an important drug target in HIV treatment. At least two isoforms of DNAL1 are known to exist.
REFERENCES: 1) Horvath J, Fliegauf M, Olbrich H, et al. Identification and analysis of axonemal dynein light chain 1 in primary ciliary dyskinesia patients. Am. J. Respir. Cell Mol. Biol.2005; 33:41-7.
2) Olbrich H, Haeffner K, Kisbert A, et al. Mutations in DNAH5 cause primary ciliary dyskinesia and randomization of left/right asymmetry. Nat. Genet.2002; 30:143-4.
3) Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

For Research Use Only