Datasheet

DNAJC13 Antibody
CATALOG NUMBER: 8109

Availability: In stock

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 249 kDa

Observed: 249 kDa
SPECIFICITY:DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.
IMMUNOGEN:DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13.

The immunogen is located within amino acids 1940 - 1990 of DNAJC13.
HOST SPECIES:Rabbit

Properties

PURIFICATION:DNAJC13 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:DNAJC13 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DNAJC13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:DNAJ homolog subfamily C member 13, receptor-mediated endocytosis 8, RME8
ACCESSION NO.:NP_056083
PROTEIN GI NO.:112421122
OFFICIAL SYMBOL:DNAJC13
GENE ID:23317

Background

BACKGROUND:DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).
REFERENCES: 1) Girard M, Poupon V, Blondeau F, et al. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J. Biol. Chem. 2005; 280:40135-43.
2) Fujibayashi A, Taguchi T, Misaki R, et al. Human RME-8 is involved in membrane trafficking through early endosomes. Cell Struct. Funct. 2008; 33:35-50.
3) Girard M and McPherson PS. RME-8 regulates trafficking of the epidermal growth factor receptor. FEBS Lett. 2008; 582:961-6.

For Research Use Only