DNAJC13 Antibody Cat. No.: 8109

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13.

The immunogen is located within amino acids 1940 - 1990 of DNAJC13.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 10-501 - Human Heart Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 249 kDa

Observed: 249 kDa

psi-iconProperties
PURIFICATION:DNAJC13 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DNAJC13 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DNAJC13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:DNAJC13
ALTERNATE NAMES:DNAJ homolog subfamily C member 13, receptor-mediated endocytosis 8, RME8
ACCESSION NO.:NP_056083
PROTEIN GI NO.:112421122
GENE ID:23317
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).
REFERENCES:1) Girard M, Poupon V, Blondeau F, et al. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J. Biol. Chem. 2005; 280:40135-43.
2) Fujibayashi A, Taguchi T, Misaki R, et al. Human RME-8 is involved in membrane trafficking through early endosomes. Cell Struct. Funct. 2008; 33:35-50.
3) Girard M and McPherson PS. RME-8 regulates trafficking of the epidermal growth factor receptor. FEBS Lett. 2008; 582:961-6.

ANTIBODIES FOR RESEARCH USE ONLY.

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