DNAJA4 Antibody Cat. No.: 8141

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (87%)
IMMUNOGEN: DNAJA4 antibody was raised against a 15 amino acid peptide near the center of human DNAJA4.

The immunogen is located within amino acids 210 - 260 of DNAJA4.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: DNAJA4 antibody can be used for detection of DNAJA4 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: DNAJA4 antibody is human, mouse, and rat reactive. At least three isoforms of DNAJA4 are known to exist; this antibody will detect all three isoforms.
POSITIVE CONTROL:1) Cat. No. 1320 - Human Colon Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 41, 44, 47 kDa

Observed: 40 kDa

psi-iconProperties
PURIFICATION:DNAJA4 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:DNAJA4 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:DNAJA4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:DNAJA4
ALTERNATE NAMES:DnaJ homolog subfamily A member 4, MST104, MSTP104, PRO1472
ACCESSION NO.:NP_061072
PROTEIN GI NO.:194328758
GENE ID:55466
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The DNAJA4 protein is the mammalian homolog of the chaperone HSP40 (1) and acts as a co-chaperone with the heat shock protein HSP70 (2). DNAJA4 is highly expressed in heart and testis, accounting for approximately 1% of total protein in mouse heart (2). DNAJA4 is also regulated by the sterol regulatory element binding transcription factor 2 (SREBF2) and plays a role in the synthesis of cholesterol (3).
REFERENCES:1) Ohtsuka K and Hata M. Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature. Cell Stress Chaperones 2000; 5:98-112.
2) Abdul KM, Terada K, Gotoh T, et al. Characterization and functional analysis of a heart-enriched DnaJ / Hsp40 homolog dj4/DjA4. Cell Stress Chaperones 2002; 7:156-66.
3) Robichon C, Varret M, Le Liepvre X, et al. DnajA4 is a SREBP-regulated chaperone involved in the cholesterol biosynthesis pathway. Biochim. Biophys. Acta 2006; 1761:1107-13.

ANTIBODIES FOR RESEARCH USE ONLY.

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